INNOVATION

October 2020

Development and intensification of NMN research on companion animals. Started joint research with Yamaguchi University Faculty of Veterinary Medicine (Prof. Toru Kimura: Biofunctional Science and Laboratory Animal Science).

Joint research has begun with Professor Toru Kimura (Joint Faculty of Veterinary Medicine, Biological Function, Experimental Zoology) of Yamaguchi University, Joint Faculty of Veterinary Medicine (Yamaguchi City, Yamaguchi Prefecture, President: Masao Oka), following on a study done in July 2019 using dogs as subjects, entitled “Longevity and Anti-aging Effects of NMN Administration on Dogs: Observation of Improvement of Blood Components and Intestinal Flora Associated with Activation of Longevity Genes.” The results obtained are going to be developed and taken one step further with this joint research.

 

In the earlier study, we observed changes in the expression levels of NAD + and SIRT1 and checked for deterioration of visceral functions in the liver and kidney, as well as hyperlipidemia, hyperglycemia, anemia, and others, and verified that by administering NMN to healthy subjects, the pre-symptomatic off-limits values approached the median values, which indicate health.

 

Canine gut flora contains good and bad bacteria, just like human gut flora. Good bacteria help maintain good health by synthesizing vitamins, assisting digestion and absorption, and activating immune functions. Bad bacteria cause intestinal degeneration, production of carcinogens and toxins, diarrhea or constipation, liver damage, lower immune resistance, cancer, hypertension, and accelerated aging. In the case of dogs, as in humans, it is known that diet, stress or aging cause good bacteria to decrease and bad bacteria to increase, disturbing the balance of the intestinal flora, which has various adverse effects on health. We expect to confirm the increase of good bacteria and the decrease of bad bacteria in the intestines.

 

In studies all around the world, it has been demonstrated that NMN is a functional substance that activates SIRT1 while suppressing age-related changes in laboratory mice. However, the action of NMN has yet to be investigated thoroughly in the field of veterinary medicine. In recent years, the lifespan of companion animals has extended, and as with humans, age-related diseases and their care have become an issue.

We are confident that the results of this study will provide new directions and indicators for maintaining pet health for the pet industry and the veterinary field.

 

Professor Kimura made the following comments regarding this research, “We planned this joint study to find out if the daily ingestion of NMN can improve the health of dogs. We expect to clarify the results that NMN directly leads to health promotion and healthy longevity of dogs. We expect that NMN will indirectly increase the flora that is related to longevity. If this result is obtained, we will be contributing to true animal welfare and the extension of their lifespan, and we will be able to live with companion animals with all the more peace of mind.”

April 2020

Launched research on NMN and immunity, prevention, and treatment of infectious diseases such as COVID-19.

Our Life Science Lab confirmed that the appropriate addition of NMN in the medium where human cells where being cultured increased both the expression of the longevity genes (also called anti-aging genes, such as sirtuin 1) at a protein level and also the number of mitochondria that convert nutrients into energy within the cells.

 

There are a vast variety of genes present in DNA that are related to sirtuin 1 and therefore involved in this process.

 

These results strongly suggest that NMN may reinforce the immune system, as it acts on the cells of the immune system itself, such as the T cells of the lymphatic system.

There are a vast variety of genes present in DNA that are related to sirtuin 1 and therefore involved in this process.

 

These results strongly suggest that NMN may reinforce the immune system, as it acts on the cells of the immune system itself, such as the T cells of the lymphatic system.

To explore how NMN could be applied for the prevention and treatment of infectious diseases, we have started experiments to observe how the functions of CD4+ helper T cells and CD8+ killer T cells, both essential for the immune system, may be influenced by NMN. We will use equipment especially designed to cultivate cells into environments that simulate different body parts where T cells work (bloodstream, capillaries, invading tissues, etc.),  and analyze the response of the T cells to NMN, including molecular mechanisms, in specific body environments.
 

While effective treatments, remedies, and preventive vaccines against the new coronavirus have not been developed yet, strengthening general immunity is an intuitive countermeasure. This means that it is important to sleep well, watch your diet, do moderate exercise, and reduce your stress levels. Although such lifestyle practices and adjustments may have a certain effect on improving immunity, lockdown restrictions make  them especially difficult to apply well.

NMN nutraceuticals are meant to work against the gradual and continuous decrease of our physical functions due to age, and developed based on analysis of the in vivo mechanisms that contribute to a healthy longevity.
 

In the midst of these ongoing efforts and facing the current pandemic of the new coronavirus, we are accelerating our research aimed at improving the immune system.
 

In addition, with the cooperation of Dr. Hiroaki Kitano, President of The Systems Biology Institute, we will work to verify the inhibitory and preventive effects on allergy, inflammation, and cytokine “storm” (severe shock-like conditions caused by a runaway immune system) focused on lung disease. Cytokine storms are strongly associated with the severity of the pneumonia caused by the new coronavirus, according to a recent paper in The Lancet, one of the world’s top five journals of medicine.
 

Also, in collaboration with medical research institutes, we will conduct new clinical trials to study NMN in relation to various parameters of immunity, such as the number of CD8+ killer T cells in the blood of humans, and various parameters related to the intestinal environment, such as species identification and number of cells present in the intestinal flora of humans.

In these clinical studies, our purpose is to measure and analyze results in detail to obtain scientific evidence for the improvement of immunity, and other potential contributors to health.

We will make the results of our research available to research institutions and the general public.


 

Conducted joint research with the Graduate School of Integrated Sciences for Life, at the National University of Hiroshima (Prof. Masayuki Shimada), on "elucidation of the effects of NMN on sperm function and fertility."

February 2020

Concluded a material donation agreement with Washington University in St. Louis (Missouri, USA).

We have concluded an agreement to donate the NMN that will be used by the Washington University in St. Louis (Missouri, USA) in a clinical study entitled “Effect of NMN Supplementation on Organ System Biology,” to be conducted on the University premises after having received a grant from the U.S. Department of Defense.

 

This clinical research, which focuses on the effects of NMN after oral administration, will be conducted by Shin-Ichiro Imai, M.D., Ph. D. (Washington University in St. Louis, Missouri), School of Medicine, Department of Developmental Biology/ Department of Medicine (joint)) and Samuel Klein (William H. Danforth Professor of Medicine and Nutritional Science Chief, Division of Geriatrics and Nutritional Science, Director of the Center for Applied Research Sciences).

December 2019

November 2019

Obtained joint patent application rights with Prof. Daiki Setoyama and Prof. Kang Dongchon of the National University of Kyushu for "Discovery of β-NMN's enhancement effect on coenzyme Q in vivo."

Study on “Longevity and Anti-Aging Effects of NMN Administration in Dogs: Confirmation of the Effects of Activation of Longevity Genes on Blood Composition and Intestinal Microbiota," conducted in collaboration with the Faculty of Veterinary Medicine, Yamaguchi University (Prof. Toru Kimura: Biofunctional Science and Laboratory Animal Science).

July 2019

November 2018

Collaboration launched with The Systems Biology Institute aimed at elucidating the biomolecular mechanisms of NMN.

There is still plenty to learn about the biomolecular mechanisms of action of nicotinamide mononucleotide (NMN), and we consider this to be a vital issue for aging research. With this in mind, and with the collaboration of the Systems Biology Institute (SBI), we have launched a series of life science research programs in order to clarify the mechanisms of action of NMN and its effects.

 

As the first step, we have concluded a research consignment (Elucidation of Intracellular Mechanism of NMN by Multi-Omics Analysis) with SBI to contribute to the science of NMN and aging research.

 

SBI’s President, Dr. Hiroaki Kitano has expressed his expectations regarding the research: "Through long-term administration, NMN is a substance expected to mitigate the physiological problems associated with aging. Although a number of physiological effects caused by the administration of NMN have been reported, the detailed biomolecular mechanisms have not yet been elucidated. This research will comprehensively analyze the effects of NMN at a cultured cell level and I hope it will be the first step leading to the next stage of research on the biomolecular and system biology of NMN."

 

◇About SBI http://www.sbi.jp/index.htm

January 2017

The world's first clinical study on the effects and mechanism of action of NMN in humans through oral ingestion (24 weeks), conducted at the Graduate School of Biomedical and Health Sciences, National University of Hiroshima.

In the final report, we confirmed an increase in the expression level of sirtuin 1 (longevity gene or anti-aging gene) and many different growth hormones. Through the increase of sirtuin 1 expression, we can expect suppression of various age-derived health problems, such as lifestyle-related diseases, cancer, dementia and other chronic diseases. While we confirmed the increase of various growth hormones, the increase of melatonin was especially remarkable. Melatonin is deeply involved in the process of sleep, the quality of which is considered to be a factor in cancer suppression.

Research Partners

Washington University School of Medicine in St. Louis

Institute for Research on Productive Aging (IRPA)

​■The Systems Biology Institute

Yamaguchi University School of Medicine Graduate School

Yamaguchi University, Joint Faculty of Veterinary Medicine

MIRAILAB BIOSCIENCE Inc. Life Science Laboratory

2-3-11 Nihonbashi Honcho, Chuo-ku, Tokyo, Japan  (Nihonbashi Life Science Building)

Staffed by one Doctor of Medicine and one Doctor of Engineering